Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals

Author:

Djaileb Abdelhadi12345,Hojjat Jodaylami Maryam16785,Coutu Julien16785,Ricard Pierre16785,Lamarre Mathieu910111213,Rochet Léa12345,Cellier-Goetghebeur Stella12345,Macaulay Devin910111213,Charron Benjamin16785,Lavallée Étienne12345,Thibault Vincent16785,Stevenson Keisean16785ORCID,Forest Simon16785,Live Ludovic S.141513,Abonnenc Nanouk16341718,Guedon Anthony16341718,Quessy Patrik16341718,Lemay Jean-François16341718,Farnós Omar19202113,Kamen Amine19202113,Stuible Matthew2223241513,Gervais Christian2223241513ORCID,Durocher Yves2223241513,Cholette François2526272813,Mesa Christine2526272813,Kim John2526272813,Cayer Marie-Pierre29301213,de Grandmont Marie-Joëlle29301213,Brouard Danny29301213,Trottier Sylvie311232,Boudreau Denis910111213ORCID,Pelletier Joelle N.12345,Masson Jean-Francois16785ORCID

Affiliation:

1. Department of Chemistry

2. Department of Biochemistry and PROTEO

3. The Québec Network for Research on Protein Function

4. Engineering and Applications

5. Université de Montréal

6. Quebec Centre for Advanced Materials (QCAM)

7. Regroupement Québécois sur les Matériaux de Pointe (RQMP)

8. and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA)

9. Department of Chemistry and Centre for Optics

10. Photonics and Lasers (COPL)

11. Université Laval

12. Québec City

13. Canada

14. Affinité Instruments

15. Montréal

16. CNETE and PROTEO

17. Cégep de Shawinigan

18. Shawinigan

19. Department of Bioengineering

20. McGill University McConnell Engineering Building

21. Montreal

22. Mammalian Cell Expression

23. Human Health Therapeutics Research Centre

24. National Research Council Canada

25. National Laboratory for HIV Reference Services

26. National Microbiology Laboratory at the JC Wilt Infectious Diseases Research Centre

27. Public Health Agency of Canada

28. Winnipeg

29. Héma-Québec

30. Affaires médicales et innovation

31. Centre de recherche du Centre hospitalier universitaire de Québec and Département de microbiologie-infectiologie et d'immunologie

32. Canada G1V 4G2

Abstract

We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for the detection of nucleocapsid and spike antibodies specific to the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and dried blood spots (DBS).

Funder

Canadian Institutes of Health Research

Natural Sciences and Engineering Research Council of Canada

Canada Foundation for Innovation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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