Development of a bioorthogonal fluorescence-based assay for assessing drug uptake and delivery in bacteria-Reference-Cited by-同舟云学术

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Development of a bioorthogonal fluorescence-based assay for assessing drug uptake and delivery in bacteria

Published:2022 Issue:25 Volume:12 Page:15631-15642
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Ooi Jocelyn M. F.¹^ORCID,Fairhall Jessica M.¹^ORCID,Spangler Benjamin²,Chong Daniel J. W.¹,Feng Brian Y.²,Gamble Allan B.¹^ORCID,Hook Sarah¹

Affiliation:

1. School of Pharmacy, University of Otago, Dunedin, New Zealand

2. Novartis Institutes for BioMedical Research (NIBR) in Emeryville, California, USA

Abstract

Bioorthogonal alkyne–azide and alkyne–tetrazine chemistries were used to assess drug uptake in bacteria. Azido-drug reacts with streptavidin bound alkyne-biotin within bacteria, the remaining unreacted alkyne is then quantified with a tetrazine-dye.

Funder

University of Otago

Health Research Council of New Zealand

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/RA/D2RA02272A

Reference52 articles.

1. A Gestalt approach to Gram-negative entry

2. Mechanisms of envelope permeability and antibiotic influx and efflux in Gram-negative bacteria

3. Physical methods to quantify small antibiotic molecules uptake into Gram-negative bacteria

4. Classification of Membrane Permeability of Drug Candidates: A Methodological Investigation

5. Predicting a Drug’s Membrane Permeability: A Computational Model Validated With in Vitro Permeability Assay Data

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