Separation and detection of free d- and l-amino acids in tea by off-line two-dimensional liquid chromatography-Reference-Cited by-同舟云学术

Separation and detection of free d- and l-amino acids in tea by off-line two-dimensional liquid chromatography

Published:2017 Issue:43 Volume:9 Page:6131-6138
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Wang Xiangyu¹²³⁴⁵,Wu Huihui⁶⁷⁵,Luo Rongying¹²³⁴⁵,Xia Donghai¹²³⁴⁵,Jiang Zhengjin¹²³⁴⁵,Han Hai¹²³⁴⁵^ORCID

Affiliation:

1. Department of Pharmacy

2. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research

3. Jinan University

4. Guangzhou 510632

5. China

6. Anhui No. 2 Province People's Hospital

7. Hefei 230041

Abstract

An off-line 2D-HPLC method was employed to achieve enantiomeric separation of amino acids in tea samples. 16 free amino acids in longjing, black, oolong, and pu-erh teas were separated and collected after FMOC-Cl derivatization in the first dimension, and the fractions were collected and concentrated and then separated on a chiral column.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/AY/C7AY01569K

Reference40 articles.

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4. Changes in antioxidant phytochemicals and volatile composition of Camellia sinensis by oxidation during tea fermentation

5. Stability, antioxidant activity and in vitro bile acid-binding of green, black and dark tea polyphenols during simulated in vitro gastrointestinal digestion

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