Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage-Reference-Cited by-同舟云学术

Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage

Published:2023 Issue:14 Volume:14 Page:3839-3851
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Sun Ruirui¹²,Zhao Yuqian¹³,Wang Wenjuan⁴,Liu Jun-Jie Gogo¹³,Chen Chunlai¹²^ORCID

Affiliation:

1. Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China

2. Beijing Frontier Research Center for Biological Structure, Tsinghua University, Beijing 100084, China

3. Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China

4. Technology Center for Protein Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China

Abstract

A positively-charged-residue enriched alpha helix in the REC2 domain serving as a conserved element to facilitate one-dimensional diffusion-driven target search of Cas12a and assisting target cleavage of AsCas12a.

Funder

National Natural Science Foundation of China

Center for Life Sciences

Beijing Advanced Innovation Center for Structural Biology, Tsinghua University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D2SC05463A

Reference72 articles.

1. CRISPR/Cas System and Its Role in Phage-Bacteria Interactions

2. CRISPR/Cas, the Immune System of Bacteria and Archaea

3. CRISPR-Mediated Adaptive Immune Systems in Bacteria and Archaea

4. RNA-guided genetic silencing systems in bacteria and archaea

5. CRISPR-CasΦ from huge phages is a hypercompact genome editor

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