Highly stable protein immobilizationviamaleimido-thiol chemistry to monitor enzymatic activity

Author:

Schartner Jonas1234,Güldenhaupt Jörn1234ORCID,Katharina Gaßmeyer Sarah5234,Rosga Katharina1234,Kourist Robert5234ORCID,Gerwert Klaus1234ORCID,Kötting Carsten1234ORCID

Affiliation:

1. Department of Biophysics

2. Ruhr-Universität Bochum

3. 44801 Bochum

4. Germany

5. Junior Research Group for Microbial Biotechnology

Abstract

Combining a novel protein immobilisation method with multivariate curve resolution enables the direct observation of biocatalysis by ATR-FTIR spectroscopy.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference54 articles.

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2. Engineering the third wave of biocatalysis

3. W. Tischer and F.Wedekind , in Biocatalysis - From Discovery to Application , ed. W.-D. Fessner , A. Archelas , D. C. Demirjian , R. Furstoss , H. Griengl , K.-E. Jaeger , E. Morís-Varas , R. Öhrlein , M. T. Reetz , J.-L. Reymond , M. Schmidt , S. Servi , P. C. Shah , W. Tischer and F. Wedekind , Springer Berlin Heidelberg , Berlin, Heidelberg , 1999 , pp. 95–126

4. Immobilization of enzymes in microtiter plate scale

5. Immobilization of (R)- and (S)-amine transaminases on chitosan support and their application for amine synthesis using isopropylamine as donor

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