The Wittig bioconjugation of maleimide derived, water soluble phosphonium ylides to aldehyde-tagged proteins-Reference-Cited by-同舟云学术

The Wittig bioconjugation of maleimide derived, water soluble phosphonium ylides to aldehyde-tagged proteins

Published:2021 Issue:47 Volume:19 Page:10417-10423
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Hartmann Rafael W.¹²^ORCID,Pijnappel Matthijs¹,Nilvebrant Johan³^ORCID,Helgudottir Hildur Run⁴^ORCID,Asbjarnarson Arni⁴,Traustadottir Gunnhildur Asta⁴^ORCID,Gudjonsson Thorarinn⁴⁵^ORCID,Nygren Per-Åke³^ORCID,Lehmann Fredrik¹^ORCID,Odell Luke R.²^ORCID

Affiliation:

1. Recipharm OT Chemistry, Virdings allé 16, 75450 Uppsala, Sweden

2. Department of Medicinal Chemistry, Uppsala University, Uppsala Biomediciniska Centrum, Husargatan 3, 75123 Uppsala, Sweden

3. Department of Protein Science, Division of Protein Engineering, KTH School of Engineering Sciences in Chemistry, Biology and Health, AlbaNova Universitetscentrum, Roslagsvägen 30B, 10961 Stockholm, Sweden

4. Stem Cell Research Unit, Biomedical Center, University of Iceland, 101 Reykjavik, Iceland

5. Department of Laboratory Hematology, Landspítali-University Hospital, Reykjavik, Iceland

Abstract

The novel bioconjugation method makes use of maleimides and tris(2-carboxyethyl)phosphine (TCEP) to conjugate small molecules to proteins via alkenes.

Funder

Uppsala Universitet

Vetenskapsrådet

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/OB/D1OB01155C

Reference44 articles.

1. Site-selective modification strategies in antibody–drug conjugates

2. Relationship Between the Occurrence of Cysteine in Proteins and the Complexity of Organisms