Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip-Reference-Cited by-同舟云学术

Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip

Published:2019 Issue:9 Volume:19 Page:1610-1620
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Biočanin Marjan¹²³⁴⁵^ORCID,Bues Johannes¹²³⁴⁵^ORCID,Dainese Riccardo¹²³⁴⁵,Amstad Esther⁶⁷⁸⁵⁹^ORCID,Deplancke Bart¹²³⁴⁵^ORCID

Affiliation:

1. Laboratory of Systems Biology and Genetics

2. Institute of Bioengineering

3. School of Life Sciences

4. Ecole Polytechnique Fédérale de Lausanne (EPFL)

5. Lausanne

6. Soft Materials Laboratory

7. Institute of Materials

8. École Polytechnique Fédérale de Lausanne (EPFL)

9. Switzerland

Abstract

Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution.

Funder

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

École Polytechnique Fédérale de Lausanne

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2019/LC/C9LC00014C

Reference28 articles.

1. The Human Cell Atlas

2. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

3. InDrops and Drop-seq technologies for single-cell sequencing

4. Droplet Microfluidics for Single-Cell Analysis

5. Droplet microfluidics for high-throughput biological assays

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