Amplification-free in situ KRAS point mutation detection at 60 copies per mL in urine in a background of 1000-fold wild type-Reference-Cited by-同舟云学术

Amplification-free in situ KRAS point mutation detection at 60 copies per mL in urine in a background of 1000-fold wild type

Published:2016 Issue:4 Volume:141 Page:1421-1433
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Kirimli Ceyhun E.¹²³⁴⁵,Shih Wei-Heng¹⁶⁵⁷,Shih Wan Y.¹²³⁴⁵

Affiliation:

1. Drexel University

2. School of Biomedical Engineering

3. Science

4. and Health Systems

5. Philadelphia

6. Department of Materials Science and Engineering

7. USA

Abstract

We have examined thein situdetection of a single-nucleotideKRASmutation in urine using a (Pb(Mg_1/3Nb_2/3)O₃)_0.65(PbTiO₃)_0.35(PMN-PT) piezoelectric plate sensor (PEPS) coated with a 17-nucleotide (nt) locked nucleic acid (LNA) probe DNA complementary to theKRASmutation.

Funder

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C5AN02048D

Reference59 articles.

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3. DNA Melting Analysis for Detection of Single Nucleotide Polymorphisms