Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes
Author:
Affiliation:
1. State Key Laboratory of Analytical Chemistry for Life Science
2. School of Chemistry and Chemical Engineering
3. Chemistry and Biomedicine Innovation Center (ChemBIC)
4. Nanjing University
5. Nanjing 210023
6. GlucoSentient
7. Champaign
8. USA
Abstract
A simple target-responsive CRISPR/Cas12a-based strategy for translating molecular detection into a glucose test.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Jiangsu Province
Fundamental Research Funds for the Central Universities
Nanjing University
Publisher
Royal Society of Chemistry (RSC)
Subject
Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00008J
Reference60 articles.
1. Advancing Point-of-Care Diagnostics of Metabolites Through Engineering Semisynthetic Proteins
2. Translating in vitro diagnostics from centralized laboratories to point-of-care locations using commercially-available handheld meters
3. Point of Care Sensors for Infectious Pathogens
4. Harnessing CRISPR Effectors for Infectious Disease Diagnostics
5. Ultrasensitive CRISPR-based diagnostic for field-applicable detection ofPlasmodiumspecies in symptomatic and asymptomatic malaria
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