Theoretical study on substrate recognition and catalytic mechanisms of gephyronic acid dehydratase DH1-Reference-Cited by-同舟云学术

Theoretical study on substrate recognition and catalytic mechanisms of gephyronic acid dehydratase DH1

Published:2021 Issue:6 Volume:11 Page:2155-2166
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Liu Lei¹²³⁴⁵,Yu Qian¹²³⁴⁵,Zhang Haoqing¹²³⁴⁵,Tao Wentao¹²³⁴⁵,Wang Rufan¹²³⁴⁵,Bai Linquan¹²³⁴⁵,Zhao Yi-Lei¹²³⁴⁵^ORCID,Shi Ting¹²³⁴⁵^ORCID

Affiliation:

1. State Key Laboratory of Microbial Metabolism

2. Joint International Research Laboratory of Metabolic and Developmental Sciences

3. School of Life Sciences and Biotechnology

4. Shanghai Jiao Tong University

5. Shanghai 200240

Abstract

The bifunctional dehydratase GphF DH1 catalyzes both the dehydration of β-hydroxy and the double bond isomerization with the energy barrier of 27.0 kcal mol⁻¹ and 17.2 kcal mol⁻¹ respectively.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shanghai

National Basic Research Program of China

Shanghai Jiao Tong University

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2021/CY/D0CY01776K

Reference41 articles.

1. Biosynthesis and chemical diversity of β-lactone natural products

2. Engineering strategies for rational polyketide synthase design

3. Engineering enzymatic assembly lines for the production of new antimicrobials

4. Engineering polyketide synthases and nonribosomal peptide synthetases

5. The Interplay between a Multifunctional Dehydratase Domain and a C-Methyltransferase Effects Olefin Shift in Ambruticin Biosynthesis

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