A comprehensive analysis of library preparation methods shows high heterogeneity of extrachromosomal circular DNA but distinct chromosomal amount levels reflecting different cell states-Reference-Cited by-同舟云学术

A comprehensive analysis of library preparation methods shows high heterogeneity of extrachromosomal circular DNA but distinct chromosomal amount levels reflecting different cell states

Published:2024 Issue:1 Volume:149 Page:148-160
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Lu Wenxiang¹^ORCID,Li Fuyu¹,Ouyang Yunfei¹,Jiang Yali²,Zhang Weizhong³,Bai Yunfei¹^ORCID

Affiliation:

1. State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, 210096, China

2. The Friendship Hospital of Ili Kazakh Autonomous Prefecture, Ili & Jiangsu Joint Institute of Health, Yining, Xinjiang Uygur Autonomous Region, 835000, China

3. Department of Ophthalmology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China

Abstract

Different cells have different distribution characteristics of eccDNA in the genomic and elements, which may be a stable feature of cells and can serve as potential biomarkers.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D3AN01300F

Reference48 articles.

1. Discoveries of Extrachromosomal Circles of DNA in Normal and Tumor Cells

2. Small extrachromosomal circular DNAs, microDNA, produce short regulatory RNAs that suppress gene expression independent of canonical promoters

3. eccDNAs are apoptotic products with high innate immunostimulatory activity

4. Extrachromosomal DNA is associated with oncogene amplification and poor outcome across multiple cancers

5. Cell-Free eccDNAs: A New Type of Nucleic Acid Component for Liquid Biopsy?