A polyA DNA probe-based ultra-sensitive and structure-distinguishable electrochemical biosensor for the analysis of RNAi transgenic maize-Reference-Cited by-同舟云学术

A polyA DNA probe-based ultra-sensitive and structure-distinguishable electrochemical biosensor for the analysis of RNAi transgenic maize

Published:2021 Issue:11 Volume:146 Page:3526-3533
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Xu Li¹²³⁴⁵,Qi Jiawei¹²³⁴⁵,Wen Yanli¹²³⁴⁵,Liang Wen¹²³⁴⁵,Wang Lele¹²³⁴⁵,Yang Zhenzhou¹²³⁴⁵,Yang Xue¹²³⁴⁵,Qi Yu¹²³⁴⁵,Duan Manlei¹²³⁴⁵,Zhao Keke¹²³⁴⁵,Gu Jie¹²³⁴⁵,Shen Yiji¹²³⁴⁵,Rao Pinhua⁶⁷⁴⁵,Ding Min¹²³⁴⁵,Ren Shuzhen¹²³⁴⁵,Li Liang⁸⁹¹⁰¹¹,Liu Gang¹²³⁴⁵^ORCID

Affiliation:

1. Laboratory of Biometrology

2. Division of Chemistry and Ionizing Radiation Measurement Technology

3. Shanghai Institute of Measurement and testing technology

4. Shanghai

5. P.R. China

6. College of Chemistry and Chemical Engineering

7. Shanghai University of Engineering Science

8. Biotechnology Research Institute

9. Chinese Academy of Agricultural Sciences

10. Beijing 100081

11. China

Abstract

A multiple reporter probe system (MRP) was applied to open the secondary structure and hybridize with a long RNA. Capability of quantification and structural analysis of long RNA was demonstrated. Excellent selectivity was achieved when analyzing extracted total RNA samples.

Funder

Youth Innovation Promotion Association of the Chinese Academy of Sciences

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00313E

Reference33 articles.

1. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

2. RNA interference