Triblock probe-polyA-probe electrochemical interfacial engineering for the sensitive analysis of RNAi plants-Reference-Cited by-同舟云学术

Triblock probe-polyA-probe electrochemical interfacial engineering for the sensitive analysis of RNAi plants

Published:2022 Issue:11 Volume:147 Page:2452-2459
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zheng Yu¹,Wang Lele¹,Xu Li¹,Li Yan¹,Yang Xue¹,Yang Zhenzhou¹,Li Lanying¹,Ding Min¹,Ren Shuzhen¹,Gong Feiyan¹,Chang Jinxue¹,Cao Chengming¹,Wen Yanli¹,Li Liang²^ORCID,Liu Gang¹^ORCID

Affiliation:

1. Key Laboratory of Bioanalysis and Metrology for State Market Regulation, Shanghai Institute of Measurement and Testing Technology, 1500 Zhang Heng Road, Shanghai 201203, People's Republic of China

2. Institute of Quality Standard and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China

Abstract

RNA interference (RNAi) is under fast development in agriculture and brings new challenge for GMO analysis. We developed a electrochemical biosensor for the analysis of GM maize samples based on a polyA-DNA capturing probe. Ultrasensitive detection of 10 fM RNA was realized.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D2AN00366J

Reference35 articles.

1. A Link Between mRNA Turnover and RNA Interference in Arabidopsis

2. A genetic link between co-suppression and RNA interference in C. elegans

3. Induction of RNA interference in Caenorhabditis elegans by RNAs derived from plants exhibiting post-transcriptional gene silencing

4. Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference

5. Engineering pest tolerance through plant-mediated RNA interference

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