Single cell-laden protease-sensitive microniches for long-term culture in 3D-Reference-Cited by-同舟云学术

Single cell-laden protease-sensitive microniches for long-term culture in 3D

Published:2017 Issue:4 Volume:17 Page:727-737
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Lienemann Philipp S.¹²³⁴⁵^ORCID,Rossow Torsten¹²³⁴⁵,Mao Angelo S.¹²³⁴⁵,Vallmajo-Martin Queralt⁶⁷⁸⁹¹⁰,Ehrbar Martin⁶⁷⁸⁹¹⁰,Mooney David J.¹²³⁴⁵

Affiliation:

1. John A. Paulson School of Engineering and Applied Sciences

2. Harvard University

3. Cambridge

4. USA

5. Wyss Institute for Biologically Inspired Engineering

6. Department of Obstetrics

7. University Hospital Zurich

8. University of Zurich

9. 8091 Zurich

10. Switzerland

Abstract

We present a powerful strategy for evading Poisson encapsulation statistics and for cell centering in single cell-laden synthetic microniches to facilitate long-term culture in protease-sensitive 3D microenvironments.

Funder

National Institutes of Health

Deutsche Forschungsgemeinschaft

Swiss National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2017/LC/C6LC01444E

Reference37 articles.

1. Microfluidic Encapsulation of Cells in Polymer Microgels

2. Stem cell niche engineering through droplet microfluidics

3. Microfluidic generation of microgels from synthetic and natural polymers

4. Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments

5. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Cited by 47 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The mechanics of the retina: Müller glia role on retinal extracellular matrix and modelling;Frontiers in Medicine;2024-09-04

2. Microfluidic Platforms to Screen Granular Hydrogel Microenvironments for Tissue Regeneration;Advanced Functional Materials;2024-05-07

3. On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process;Biofabrication;2024-03-28

4. Unveiling the Potential of Single‐Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives;Small Science;2024-03-25

5. Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications;Bioengineering & Translational Medicine;2023-12-04