Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics
Author:
Affiliation:
1. School of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience
2. Georgia Institute of Technology
3. Atlanta
4. USA
Abstract
Here reduction and alkylation with commonly used reagents were systematically evaluated and optimized for MS-based bottom-up proteomics.
Funder
Division of Chemistry
National Institute of General Medical Sciences
Publisher
Royal Society of Chemistry (RSC)
Subject
Molecular Biology,Biotechnology
Link
http://pubs.rsc.org/en/content/articlepdf/2017/MB/C7MB00393E
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