Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response
Author:
Affiliation:
1. Department of Chemistry
2. University of Alberta
3. Edmonton, Canada
4. Department of Physiology
Abstract
We describe the use of μFACS to aid the directed evolution of a genetically encoded yellow fluorescent Ca2+indicator.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry,Biophysics
Link
http://pubs.rsc.org/en/content/articlepdf/2014/IB/C4IB00039K
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