Stochastic assembly of biomacromolecular complexes: impact and implications on charge interpretation in native mass spectrometry-Reference-Cited by-同舟云学术

Stochastic assembly of biomacromolecular complexes: impact and implications on charge interpretation in native mass spectrometry

Published:2023 Issue:35 Volume:14 Page:9316-9327
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Yin Victor¹²^ORCID,Devine Paul W. A.³,Saunders Janet C.⁴,Barendregt Arjan¹²,Cusdin Fiona⁴,Ristani Alexandra⁴,Hines Alistair³,Shepherd Sam³,Dembek Marcin⁵^ORCID,Dobson Claire L.⁴,Snijder Joost¹²^ORCID,Bond Nicholas J.³,Heck Albert J. R.¹²^ORCID

Affiliation:

1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

2. Netherlands Proteomics Center, Padualaan 8, 3584 CH, Utrecht, The Netherlands

3. Analytical Sciences, Biopharmaceutical Development, R & D, AstraZeneca, Granta Park, Cambridge, UK

4. In Vivo Expressed Biologics, Discovery Sciences, R & D, AstraZeneca, Granta Park, Cambridge, UK

5. Purification Process Sciences, Biopharmaceutical Development, R & D, AstraZeneca, Granta Park, Cambridge, UK

Abstract

An interpretative framework for analyzing by spectral simulations high resolution native mass spectra of protein assemblies is proposed. It reveals that the spectral appearances of heterogeneous assemblies can be misleadingly simple.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

AstraZeneca

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D3SC03228K

Reference44 articles.

1. Native Mass Spectrometry: What is in the Name?

2. Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry

3. High-Resolution Native Mass Spectrometry

4. Native Mass Spectrometry: Recent Progress and Remaining Challenges

5. Characterizing Endogenous Protein Complexes with Biological Mass Spectrometry

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