A minimal standardized human bone marrow microphysiological system to assess resident cell behavior during normal and pathological processes

Author:

Voeltzel Thibault12345ORCID,Fossard Gaëlle12346,Degaud Michaël12346ORCID,Geistlich Kevin12347,Gadot Nicolas1238,Jeanpierre Sandrine12347,Mikaelian Ivan1234,Brevet Marie9,Anginot Adrienne105,Le Bousse-Kerdilès Marie-Caroline105,Trichet Valérie115,Lefort Sylvain12345ORCID,Maguer-Satta Véronique12345ORCID

Affiliation:

1. CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 69000 Lyon, France

2. Inserm U1052, Centre de Recherche en Cancérologie de Lyon, 69000 Lyon, France

3. Université de Lyon, 69000, Lyon, France

4. Department of Cancer Initiation and Tumor cell Identity and Lyon, France

5. CNRS GDR 3697 MicroNiT, Tours, France

6. Hospices Civils de Lyon, Hematology Department, Centre Hospitalier Lyon Sud, F-69495 Pierre Bénite, France

7. Centre Léon Bérard, Lyon, France

8. Research Pathology Platform, Department of Translational Research and Innovation, Centre Léon Bérard, Lyon, France

9. Pathology Department, Hospices Civils de Lyon, Bron F-69500, France

10. UMR1197, Université Paris-Saclay, 94800 Villejuif, France

11. INSERM, UMR 1238, PHYOS, Faculty of Medicine, University of Nantes, Nantes, France

Abstract

We provide an easy to access microphysiological standardized system approaching the human bone marrow complexity to a first level of analysis by in situ imaging or by viable cell harvesting of processes taking place within this ecosystem.

Funder

Ligue Contre le Cancer

Fondation de France

Institut National Du Cancer

Agence Nationale de la Recherche

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

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