Specific recognition and sensitive quantification of mRNA splice variants <i>via</i> one-pot ligation-dependent loop-mediated isothermal amplification-Reference-Cited by-同舟云学术

Specific recognition and sensitive quantification of mRNA splice variants via one-pot ligation-dependent loop-mediated isothermal amplification

Published:2023 Issue:22 Volume:148 Page:5605-5611
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhang Mai¹,Wang Hui¹^ORCID,Han Jun²,Wang Honghong¹^ORCID,Jia Yuting¹,Hong Weixiang¹,Tang Fu³^ORCID,Li Zhengping¹^ORCID

Affiliation:

1. Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China

2. National Textile and Leather Product Quality Inspection and Testing Centre, 15 Xili-Balizhuang, Chaoyang District, Beijing 100025, China

3. School of Materials Science and Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China

Abstract

In this work, by ingeniously designing the splicing junction-specific stem-loop DNA probe pair, we proposed a one-pot ligation-dependent loop-mediated isothermal amplification strategy (one-pot ligation-LAMP) for detecting splicing variants.

Funder

Fundamental Research Funds for the Central Universities

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN01382K

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