An integrated microfluidic device for multiplexed imaging of spatial gene expression patterns of <i>Drosophila</i> embryos-Reference-Cited by-同舟云学术

An integrated microfluidic device for multiplexed imaging of spatial gene expression patterns of Drosophila embryos

Published:2022 Issue:21 Volume:22 Page:4081-4092
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Zhu Hongcun¹,Shen Wenting²,Luo Chunxiong¹²³^ORCID,Liu Feng²⁴^ORCID

Affiliation:

1. The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China

2. Center for Quantitative Biology, Peking University, Beijing, 100871, China

3. Wenzhou Institute University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China

4. Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, School of Health Science & Biomedical Engineering, Hebei University of Technology, Tianjin, 300130, China

Abstract

An integrated microfluidic device to achieve embryo trapping with orientation selectivity and sequential imaging on multiple protein spatial profiles of Drosophila embryos via n cycles of immunofluorescence staining and de-staining.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D2LC00514J

Reference68 articles.

1. Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

2. MorphoSeq: Full Single-Cell Transcriptome Dynamics Up to Gastrulation in a Chordate

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5. Single-Cell and Single-Molecule Analysis of Gene Expression Regulation

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