Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites-Reference-Cited by-同舟云学术

Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

Published:2020-12-11 Issue:6522 Volume:370 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Veenvliet Jesse V.¹^ORCID,Bolondi Adriano²^ORCID,Kretzmer Helene²^ORCID,Haut Leah¹²,Scholze-Wittler Manuela¹,Schifferl Dennis¹,Koch Frederic¹,Guignard Léo³^ORCID,Kumar Abhishek Sampath²,Pustet Milena¹,Heimann Simon¹^ORCID,Buschow René⁴^ORCID,Wittler Lars¹,Timmermann Bernd⁵,Meissner Alexander²⁶⁷⁸^ORCID,Herrmann Bernhard G.¹⁹^ORCID

Affiliation:

1. Department of Developmental Genetics, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

2. Department of Genome Regulation, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

3. Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, 10115 Berlin, Germany.

4. Microscopy and Cryo-Electron Microscopy, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

5. Sequencing Core Facility, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

6. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.

7. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

8. Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.

9. Institute for Medical Genetics, Charité–University Medicine Berlin, Campus Benjamin Franklin, 12203 Berlin, Germany.

Abstract

Trunk formation in a dish Building mammalian embryos from self-organizing stem cells in culture would accelerate the investigation of morphogenetic and differentiation processes that shape the body plan. Veenvliet et al. report a method for generating embryonic trunk-like structures (TLSs) with a neural tube, somites, and gut by embedding mouse embryonic stem cell aggregates in an extracellular matrix surrogate. Live imaging and comparative single-cell transcriptomics indicate that TLS formation is analogous to mouse development. TLSs therefore provide a scalable, tractable, and accessible high-throughput platform for decoding mammalian embryogenesis at a high level of resolution. Science , this issue p. eaba4937

Funder

Max Planck Society

NIH

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference71 articles.

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5. Role of the extracellular matrix in morphogenesis

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