Kinetic analysis of an enzymatic hydrolysis of p-nitrophenyl acetate with carboxylesterase by pressure-assisted capillary electrophoresis/dynamic frontal analysis

Author:

Mine Masanori1234,Matsumoto Naoya5234,Mizuguchi Hitoshi6234ORCID,Takayanagi Toshio6234ORCID

Affiliation:

1. Graduate School of Advanced Technology and Science

2. Tokushima University

3. Tokushima 770-8506

4. Japan

5. Faculty of Science and Technology

6. Graduate School of Technology, Industrial and Social Sciences

Abstract

An enzymatic hydrolysis of p-nitrophenyl acetate with carboxylesterase was analyzed by capillary electrophoresis/dynamic frontal analysis (CE/DFA).

Funder

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference42 articles.

1. N. V. Bhagavan and C.-E.Ha , in Essentials of Medical Biochemistry with Clinical Cases, Enzyme and Enzyme Regulation , Academic Press , London UK , 2011 , ch. 6, pp. 47–58

2. Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

3. Determination of minute enzymatic activities by means of capillary electrophoretic techniques

4. Advances in-capillary electrophoretic enzyme assays

5. Ultramicro enzyme assays in a capillary electrophoretic system

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