The effect of charge alteration and flexibility on the function and structural stability of sweet-tasting brazzein-Reference-Cited by-同舟云学术

The effect of charge alteration and flexibility on the function and structural stability of sweet-tasting brazzein

Published:2016 Issue:64 Volume:6 Page:59834-59841
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Jafari S. Shahrbanoo¹²³⁴⁵,Jafarian Vahab¹²³⁴⁵,Khalifeh Khosrow⁶²³⁴⁵,Ghanavatian Parisa¹²³⁴⁵,Shirdel S. Akram¹²³⁴⁵

Affiliation:

1. Laboratory of Biochemistry

2. Department of Biology

3. Faculty of Sciences

4. University of Zanjan

5. Zanjan

6. Laboratory of Biophysics

Abstract

To identify the structural and functional roles of Glu⁹ located at the first loop of sweet-tasting brazzein, two different mutants (E9K and E9G) of the minor form of brazzein were designed and constructed.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/RA/C6RA12626J

Reference30 articles.

1. Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli

2. Sweet proteins – Potential replacement for artificial low calorie sweeteners

3. Hypothesis/review: The structural basis of sweetness perception of sweet-tasting plant proteins can be deduced from sequence analysis

4. Critical regions for the sweetness of brazzein 1

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