FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants-Reference-Cited by-同舟云学术

FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants

Published:2020 Issue:69 Volume:56 Page:10038-10041
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Wang Junyan¹²³⁴⁵,Bing Tao¹²³⁴⁵^ORCID,Zhang Nan¹²³⁴⁵,Liu Xiangjun¹²³⁴⁵^ORCID,Shangguan Dihua¹²³⁴⁵^ORCID

Affiliation:

1. Beijing National Laboratory for Molecular Sciences

2. Key Laboratory of Analytical Chemistry for Living Biosystems

3. CAS, Research/Education Center for Excellence in Molecular Sciences

4. Institute of Chemistry

5. Chinese Academy of Sciences

Abstract

A new FnCas12a/crRNA assisted Dumbbell-PCR (Cas-Db-PCR) method is developed for the detection of isomiR heterogeneity in length and/or sequence variants with high specificity and sensitivity.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2020/CC/D0CC04348F

Reference26 articles.

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2. MicroRNA signatures in human cancers

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4. The Exoribonuclease Nibbler Controls 3′ End Processing of MicroRNAs in Drosophila

5. Structural basis of microRNA length variety

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