Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria-Reference-Cited by-同舟云学术

Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria

Published:2021 Issue:31 Volume:13 Page:13538-13549
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Obořilová Radka¹²,Šimečková Hana³,Pastucha Matěj¹²^ORCID,Klimovič Šimon¹²,Víšová Ivana⁴^ORCID,Přibyl Jan²^ORCID,Vaisocherová-Lísalová Hana⁴,Pantůček Roman³^ORCID,Skládal Petr¹²^ORCID,Mašlaňová Ivana³,Farka Zdeněk¹²^ORCID

Affiliation:

1. Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic

2. CEITEC MU, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic

3. Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

4. Institute of Physics of the CAS, Na Slovance 1999/2, 182 21 Prague, Czech Republic

Abstract

A novel methodology that uses AFM and SPR was developed for monitoring the dynamics of bacterial lysis. The lysis of S. aureus cells mediated by the enzyme lysostaphin and Podoviridae phage P68 was studied under native conditions in real-time.

Funder

Ministerstvo Školství, Mládeže a Tělovýchovy

Grantová Agentura České Republiky

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2021/NR/D1NR02921E

Reference66 articles.

1. Multidrug Resistance in Bacteria

2. Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review