Automatic and multi-channel detection of bacteria on a slidable centrifugal disc based on FTA card nucleic acid extraction and recombinase aided amplification-Reference-Cited by-同舟云学术

Automatic and multi-channel detection of bacteria on a slidable centrifugal disc based on FTA card nucleic acid extraction and recombinase aided amplification

Published:2022 Issue:1 Volume:22 Page:80-89
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Wang Siyuan¹²,Cai Gaozhe³,Duan Hong¹,Qi Wuzhen²,Lin Jianhan¹²^ORCID

Affiliation:

1. Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China

2. Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China

3. State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China

Abstract

This study developed a slidable centrifugal disc for automatic and multi-channel detection of Salmonella using FTA card for DNA extraction and RAA for DNA detection and verified it could detect 10 CFU mL−1 in chicken meat supernatant in 1 h.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D1LC00915J

Reference36 articles.

1. Investigation of Class I Integron in Salmonella infantis and Its Association With Drug Resistance

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3. Isolation, structural characterization, and lymphopoiesis stimulant activity of a polysaccharide from the abalone gonad

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