Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform-Reference-Cited by-同舟云学术

Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform

Published:2016 Issue:8 Volume:16 Page:1447-1456
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Huang Shih-Hsuan¹²³⁴,Hung Lien-Yu¹²³⁴,Lee Gwo-Bin¹²³⁴⁵

Affiliation:

1. Department of Power Mechanical Engineering

2. National Tsing Hua University

3. Hsinchu 30013

4. Republic of China

5. Institute of Biomedical Engineering

Abstract

An innovative, microfluidics-based approach featuring optically-induced cell lysis (OICL) for nucleus extraction and collection in an automatic format was demonstrated. The efficiency of cell membrane lysis and the ODEP nucleus separation was measured to be 78.04 ± 5.70% and 80.90 ± 5.98%, respectively, leading to an overall nucleus extraction efficiency of 58.21 ± 2.21%.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2016/LC/C5LC01284H

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