Uracil-DNA-glycosylase-assisted loop-mediated isothermal amplification for detection of bacteria from urine samples with reduced contamination-Reference-Cited by-同舟云学术

Uracil-DNA-glycosylase-assisted loop-mediated isothermal amplification for detection of bacteria from urine samples with reduced contamination

Published:2020 Issue:21 Volume:145 Page:7048-7055
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zeng Yingmin¹²³⁴,Liu Meiling¹²³⁴,Xia Yong¹²³⁴,Jiang Xingyu⁵⁶⁷⁴⁸^ORCID

Affiliation:

1. Department of Clinical Laboratory

2. Third Affiliated Hospital of Guangzhou Medical University

3. Guangzhou

4. People's Republic of China

5. Department of Biomedical Engineering

6. Southern University of Science and Technology

7. Shenzhen

8. Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety

Abstract

Urine specimens are detected by conventional culture method and colonies with more than 10⁴ are identified by MALDI-TOF MS. Meanwhile, we analyze urine samples using FTA cards for simple DNA extraction and UDG-assisted LAMP.

Funder

National Basic Research Program of China

National Natural Science Foundation of China

Chinese Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/D0AN01001D

Reference44 articles.

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