Reporter emission multiplexing in digital PCRs (REM-dPCRs): direct quantification of multiple target sequences per detection channel by population specific reporters-Reference-Cited by-同舟云学术

Reporter emission multiplexing in digital PCRs (REM-dPCRs): direct quantification of multiple target sequences per detection channel by population specific reporters

Published:2023 Issue:20 Volume:148 Page:5243-5254
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Calabrese Silvia¹^ORCID,Markl Anja M.¹²,Neugebauer Maximilian¹³,Krauth Stefanie J.⁴⁵,Borst Nadine¹³^ORCID,von Stetten Felix¹³^ORCID,Lehnert Michael¹^ORCID

Affiliation:

1. Hahn-Schickard, 79110 Freiburg, Germany

2. Faculty of Chemistry and Pharmacy, Albert-Ludwigs-Universität Freiburg, 79104 Freiburg, Germany

3. Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, 79110 Freiburg, Germany

4. School of Biodiversity, One Health, and Veterinary Medicine, University of Glasgow, Glasgow, UK

5. School of Health and Wellbeing, General Practice and Primary Care, University of Glasgow, Glasgow, UK

Abstract

Concept of reporter emission multiplexing (REM) to increase multiplexing capacities in digital PCR by using population specific reporters (PSR) labeled with fluorophores of different signal intensities.

Funder

Bundesministerium für Bildung und Forschung

Ministerium für Wirtschaft, Arbeit und Wohnungsbau Baden-Württemberg

Allianz Industrie Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN00191A

Reference30 articles.

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2. Clinical Utility of Liquid Biopsy-Based Actionable Mutations Detected via ddPCR

3. Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients

4. Digital PCR applications for the diagnosis and management of infection in critical care medicine

5. Centrifugal Microfluidic Integration of 4-Plex ddPCR Demonstrated by the Quantification of Cancer-Associated Point Mutations

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