A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip

Author:

Mao Ping123,Cao Lei24,Li Zedong24,You Minli24,Gao Bin5,Xie Xianghong3,Xue Zhenrui12,Peng Ping12,Yao Chunyan1,Xu Feng24ORCID

Affiliation:

1. Department of Transfusion Medicine, Southwest Hospital, Third Military Medical University, Army Medical University, Chongqing 400038, P.R. China

2. Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P.R. China

3. Department of Clinical Laboratory, Sichuan Provincial Crops Hospital, Chinese People's Armed Police Forces, Leshan 614000, P.R. China

4. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China

5. Department of Endocrinology and Metabolism, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China

Abstract

A surface patterned chip for pump-free sample digitalization and open droplet array formation used for digital isothermal nucleic acid testing.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shaanxi Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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