Decellularization of porcine kidney with submicellar concentrations of SDS results in the retention of ECM proteins required for the adhesion and maintenance of human adult renal epithelial cells-Reference-Cited by-同舟云学术

Decellularization of porcine kidney with submicellar concentrations of SDS results in the retention of ECM proteins required for the adhesion and maintenance of human adult renal epithelial cells

Published:2022 Issue:11 Volume:10 Page:2972-2990
ISSN:2047-4830
Container-title:Biomaterials Science
language:en
Short-container-title:Biomater. Sci.

Author:

Bongolan Tonya¹²,Whiteley Jennifer²,Castillo-Prado Jorge²,Fantin Amanda¹²,Larsen Brett²,Wong Cassandra J.²,Mazilescu Laura³⁴^ORCID,Kawamura Masataka³⁴^ORCID,Urbanellis Peter³⁴,Jonebring Anna⁵,Salter Eric¹²,Collingridge Graham¹²,Gladdy Rebecca²⁶,Hicks Ryan⁷,Gingras Anne-Claude²⁸,Selzner Markus³⁴,Rogers Ian M.¹²⁴⁹^ORCID

Affiliation:

1. Department of Physiology, University of Toronto, Toronto, ON, M5S 1A8, Canada

2. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada

3. Toronto General Hospital Research Institute, Toronto, ON, M5G 2C4, Canada

4. Soham & Shaila Ajmera Family Transplant Centre, University Health Network, Toronto, ON, M5G 2C4, Canada

5. Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Gothenburg, 431 83, Sweden

6. Institute of Medical Science, University of Toronto, Toronto, ON, M5S1A8, Canada

7. BioPharmaceuticals R&D Cell Therapy Department, Research and Early Development, Cardiovascular, Renal, and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, 431 83, Sweden

8. Department of Molecular Genetics, University of Toronto, Toronto, ON, M5G1E2, Canada

9. Department of Obstetrics and Gynecology, University of Toronto, Toronto, ON, M5G1E2, Canada

Abstract

When decellularizing kidneys, it is important to maintain the integrity of the acellular ECM, including associated adhesion proteins and growth factors that allow recellularized cells to adhere and migrate according to ECM specificity.

Funder

Ontario Research Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/BM/D1BM01017D

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