Isothermal cross-boosting extension–nicking reaction mediated exponential signal amplification for ultrasensitive detection of polynucleotide kinase
Author:
Affiliation:
1. State Key Laboratory of Medicinal Chemical Biology
2. Tianjin Key Laboratory of Biosensing and Molecular Recognition
3. Research Center for Analytical Sciences
4. College of Chemistry
5. Nankai University
Abstract
A novel nucleic acid-based isothermal signal amplification strategy, named cross-boosting extension–nicking reaction (CBENR) is developed and successfully used for rapid and ultrasensitive detection of polynucleotide kinase (PNK) activity.
Funder
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
Publisher
Royal Society of Chemistry (RSC)
Subject
Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2020/AN/C9AN02569C
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