Phosphorothioated and phosphate-terminal dumbbell (PP-TD) probe-based rapid detection of polynucleotide kinase activity-Reference-Cited by-同舟云学术

Phosphorothioated and phosphate-terminal dumbbell (PP-TD) probe-based rapid detection of polynucleotide kinase activity

Published:2022 Issue:22 Volume:147 Page:4986-4990
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Jiang Xianfeng¹,Shen Xudan²^ORCID,Talap Jadera²,Yang Dan²,Zeng Su²,Liu Hui¹,Cai Sheng²^ORCID

Affiliation:

1. Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310020, China

2. Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Science, Zhejiang University, Hangzhou, Zhejiang 310058, China

Abstract

A primer-free, sensitive assay has been developed to detect polynucleotide kinase (PNK) activity. This proposed method provides a promising platform for PNK activity monitoring and inhibition screening for drug discovery and clinical treatment.

Funder

Natural Science Foundation of Zhejiang Province

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D2AN01431A

Reference32 articles.

1. The threat of programmed DNA damage to neuronal genome integrity and plasticity

2. Mutations in PNKP Cause Recessive Ataxia with Oculomotor Apraxia Type 4

3. Expanding the spectrum of genes responsible for hereditary motor neuropathies

4. Mutations in PNKP cause microcephaly, seizures and defects in DNA repair

5. Pathological mutations in PNKP trigger defects in DNA single-strand break repair but not DNA double-strand break repair

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