A fluorescent methylation-switchable probe for highly sensitive analysis of FTO N6-methyladenosine demethylase activity in cells-Reference-Cited by-同舟云学术

A fluorescent methylation-switchable probe for highly sensitive analysis of FTO N6-methyladenosine demethylase activity in cells

Published:2018 Issue:36 Volume:9 Page:7174-7185
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Cheong Adeline¹²³,Low Joanne J. A.¹²³,Lim Andrea¹²³⁴⁵,Yen Paul M.⁴⁵³,Woon Esther C. Y.¹²³^ORCID

Affiliation:

1. Department of Pharmacy

2. National University of Singapore

3. Singapore

4. Program of Cardiovascular and Metabolic Disorders

5. Duke-NUS Medical School

Abstract

A dynamic biosensor which switches conformation according to its methylation status enables highly sensitive detection of m⁶A-demethylase activity. This strategy may be adapted to a broad range of RNA-modifying enzymes.

Funder

Singapore Ministry of Health's National Medical Research Council

Singapore Ministry of Education

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/SC/C8SC02163E

Reference63 articles.

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4. Gene expression regulation mediated through reversible m6A RNA methylation

5. The dynamic epitranscriptome: N6-methyladenosine and gene expression control

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