Digital droplet PCR on disk-Reference-Cited by-同舟云学术

Digital droplet PCR on disk

Published:2016 Issue:1 Volume:16 Page:208-216
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Schuler Friedrich¹²³⁴⁵,Trotter Martin¹²³,Geltman Marcel¹²³,Schwemmer Frank⁴⁵⁶²³,Wadle Simon¹²³⁴⁵,Domínguez-Garrido Elena⁷⁸⁹¹⁰,López María⁷⁸⁹¹⁰,Cervera-Acedo Cristina⁷⁸⁹¹⁰,Santibáñez Paula⁷⁸⁹¹⁰,von Stetten Felix¹²³⁴⁵,Zengerle Roland¹²³⁴⁵,Paust Nils¹²³⁴⁵

Affiliation:

1. Hahn-Schickard

2. 79110 Freiburg

3. Germany

4. Laboratory for MEMS Applications

5. IMTEK – Department of Microsystems Engineering

6. University of Freiburg

7. Molecular Diagnostics Laboratory

8. Rioja Salud-Foundation

9. La Rioja

10. Spain

Abstract

An integrated digital droplet PCR system is presented that enables droplet generation, PCR amplification, and readout within a single reaction chamber on a centrifugal microfluidic LabDisk.

Funder

European Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2016/LC/C5LC01068C

Reference29 articles.

1. Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

2. Droplet microfluidics for high-throughput biological assays

3. Digital PCR on a SlipChip