An engineered T7 RNA polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis-Reference-Cited by-同舟云学术

An engineered T7 RNA polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis

Published:2024 Issue: Volume:252 Page:431-449
ISSN:1359-6640
Container-title:Faraday Discussions
language:en
Short-container-title:Faraday Discuss.

Author:

Miller Mathew¹^ORCID,Alvizo Oscar¹^ORCID,Baskerville Scott¹^ORCID,Chintala Avinash²^ORCID,Chng Chinping¹^ORCID,Dassie Justin¹^ORCID,Dorigatti Jonathan¹^ORCID,Huisman Gjalt¹^ORCID,Jenne Stephan¹^ORCID,Kadam Supriya¹^ORCID,Leatherbury Neil²^ORCID,Lutz Stefan¹^ORCID,Mayo Melissa¹^ORCID,Mukherjee Arpan²^ORCID,Sero Antoinette¹^ORCID,Sundseth Stuart²^ORCID,Penfield Jonathan¹^ORCID,Riggins James¹^ORCID,Zhang Xiyun¹^ORCID

Affiliation:

1. Codexis, Inc., 200 Penobscot Drive, Redwood City, CA 94063, USA

2. Precision Biosciences, 302 East Pettigrew St, Durham, NC 27701, USA

Abstract

We have engineered a novel RNA polymerase, T7-68, that improves co-transcriptional incorporation of selected cap analogs and reduces dsRNA content, addressing manufacturing challenges and facilitating large-scale mRNA production.

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/FD/D4FD00023D

Reference41 articles.

1. The paradigm shift in treatment from Covid-19 to oncology with mRNA vaccines

2. BNT162b2 and mRNA-1273 COVID-19 vaccine effectiveness against the SARS-CoV-2 Delta variant in Qatar

3. mRNA capping: biological functions and applications

4. The promise of mRNA vaccines: a biotech and industrial perspective

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