Sensing platform for nucleic-acid detection based on a 2-aminopurine probe sheared by trans-cleavage activity of the CRISPR/Cas12a system-Reference-Cited by-同舟云学术

Sensing platform for nucleic-acid detection based on a 2-aminopurine probe sheared by trans-cleavage activity of the CRISPR/Cas12a system

Published:2023 Issue:11 Volume:148 Page:2482-2492
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Chen Xiaolong¹^ORCID,Huang Chaowang¹,Hu Qiao¹,Zhang Jing¹,Wang Dan¹,You Qianyi¹,Hu Mingdong¹²

Affiliation:

1. Department of Geriatrics and Special Services Medicine, Xinqiao Hospital, Army Military Medical University, Chongqing 400037, China

2. Department of Health Management, Xinqiao Hospital, Army Military Medical University, Chongqing 400037, China

Abstract

A 2-AP probe is used to replace ssDNA-FQ in the classical CRISPR/Cas12 system, realizing the highly sensitive detection of nucleic acids by the CRISPR/Cas12 system with the screened 2-AP probe as a reporter.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN00502J

Reference32 articles.

1. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

2. CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

3. Advances and insights in the diagnosis of viral infections

4. The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design (review)

5. CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity

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