Interfacing centrifugal microfluidics with linear-oriented 8-tube strips and multichannel pipettes for increased throughput of digital assays-Reference-Cited by-同舟云学术

Interfacing centrifugal microfluidics with linear-oriented 8-tube strips and multichannel pipettes for increased throughput of digital assays

Published:2023 Issue:11 Volume:23 Page:2623-2632
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Lai Yu-Kai¹²^ORCID,Kao Yu-Ting²^ORCID,Hess Jacob Friedrich¹²^ORCID,Calabrese Silvia¹^ORCID,von Stetten Felix¹²^ORCID,Paust Nils¹²^ORCID

Affiliation:

1. Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany

2. Laboratory for MEMS Applications, IMTEK – Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany

Abstract

We present a centrifugal microfluidic cartridge for the eight-fold parallel generation of monodisperse water-in-oil droplets using standard laboratory equipment.

Funder

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2023/LC/D3LC00339F

Reference49 articles.

1. Digital Assays Part I: Partitioning Statistics and Digital PCR

2. Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria

3. Single Molecule Protein Detection with Attomolar Sensitivity Using Droplet Digital Enzyme-Linked Immunosorbent Assay

4. Ultrarapid Generation of Femtoliter Microfluidic Droplets for Single-Molecule-Counting Immunoassays

5. A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate

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