An improved, optimised and robust keratin azure assay for accurate assessment of keratinase activity-Reference-Cited by-同舟云学术

An improved, optimised and robust keratin azure assay for accurate assessment of keratinase activity

Published:2023 Issue:46 Volume:15 Page:6468-6475
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Cowan Rhona M.¹^ORCID,Birch Eleanor¹,Nisbet Grace¹,Onyeiwu Chimaeze²,Campbell Clare³,Archer Ian⁴,Campopiano Dominic J.¹^ORCID

Affiliation:

1. School of Chemistry, University of Edinburgh, David Brewster Road, King's Buildings, Edinburgh, EH9 3FJ, UK

2. Johnstons of Elgin, Newmill, Elgin, Moray, Elgin, IV30 4AF, UK

3. Prickly Thistle Scotland Ltd, Evanton Industrial Estate, Beechwood Rd, Evanton, Alness IV16 9XJ, UK

4. Industrial Biotechnology Innovation Centre (IBioIC), Inovo Building, 121 George Street, Glasgow G1 1RD, UK

Abstract

Keratin from sheep's wool is an undervalued, natural resource which could be valourised if treated with a keratinase biocatalyst. We describe an improved method for the accurate quantification of keratinase activity using an azure dyed keratin.

Funder

Industrial Biotechnology Innovation Centre

University of Edinburgh

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AY/D3AY01433A

Reference35 articles.

1. The Structure, Functions, and Mechanical Properties of Keratin

2. UKRI Innovate UK , Wool Innovation Action Plan to Support a Circular and Sustainable Future , https://iuk.ktn-uk.org/wp-content/uploads/2023/05/Wool-Innovation-Action-Plan.pdf , (accessed June 2023)

3. Keratin Waste Recycling Based on Microbial Degradation: Mechanisms and Prospects

4. C. R.Robbins , Chemical and Physical Behaviour of Human Hair , Springer , Berlin , 5th edn, 2012 , ch. 1, pp. 1–104

5. Comparative study on the effects of superheated water and high temperature alkaline hydrolysis on wool keratin

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Keratinous and corneous-based products towards circular bioeconomy: A research review;Environmental Science and Ecotechnology;2024-11

2. Design, development and characterization of a chimeric protein with disulfide reductase and protease domain showing keratinase activity;International Journal of Biological Macromolecules;2024-10