Development of a single-step fluorogenic sirtuin assay and its applications for high-throughput screening-Reference-Cited by-同舟云学术

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Development of a single-step fluorogenic sirtuin assay and its applications for high-throughput screening

Published:2022 Issue:6 Volume:20 Page:1243-1252
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Wang Pan¹,Chen Di¹,An Jian-xiong¹,Lin Shu-xian¹,Liu Ting¹,Li Yan²,Chen Lei¹,He Bin¹^ORCID

Affiliation:

1. State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Provincial Key Laboratory of Pharmaceutics, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China

2. School of Basic Medical Science, Guizhou Medical University, Guiyang, Guizhou 50004, China

Abstract

A single-step fluorogenic sirtuin assay with the spontaneous intramolecular transesterification has been developed for high-throughput screening.

Funder

Guizhou Science and Technology Department

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/OB/D1OB02347K

Reference42 articles.

1. The Sir2 Family of Protein Deacetylases

2. The Substrate Specificity of Sirtuins

3. Phylogenetic Classification of Prokaryotic and Eukaryotic Sir2-like Proteins

4. Sirtuins, epigenetics and longevity

5. Sirtuin Inhibition: Strategies, Inhibitors, and Therapeutic Potential

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A homogeneous time-resolved fluorescence screen to identify SIRT2 deacetylase and defatty-acylase inhibitors;PLOS ONE;2024-06-24

2. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging;Archives of Toxicology;2023-08-19

3. Why Is Longevity Still a Scientific Mystery? Sirtuins—Past, Present and Future;International Journal of Molecular Sciences;2022-12-31

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