Development of a novel bacteriophage based biomagnetic separation method as an aid for sensitive detection of viable Escherichia coli-Reference-Cited by-同舟云学术

Development of a novel bacteriophage based biomagnetic separation method as an aid for sensitive detection of viable Escherichia coli

Published:2016 Issue:3 Volume:141 Page:1009-1016
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Wang Ziyuan¹²³,Wang Danhui¹²³,Chen Juhong¹²³,Sela David A.¹²³,Nugen Sam R.¹²³

Affiliation:

1. University of Massachusetts

2. Amherst

3. USA

Abstract

Genetic engineering of bacteriophage T7 allowed thein vivobiotinylation of capsid proteins. Oriented immobilization of the phage on magnetic beads then enabled the adsorption and separation ofE. colifrom liquid samples.

Funder

China Scholarship Council

Center for Produce Safety

Center for Hierarchical Manufacturing, National Science Foundation

National Institute of Food and Agriculture

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C5AN01769F

Reference46 articles.

1. Application of an automated immunomagnetic separation–enzyme immunoassay for the detection of Salmonella enterica subspecies enterica from poultry environmental swabs

2. Application of a novel immunomagnetic separation–bacteriophage assay for the detection of Salmonella enteritidis and Escherichia coli O157:H7 in food

3. Isolation and detection of Campylobacter jejuni from chicken fecal samples by immunomagnetic separation–PCR

4. Optimizing bacteriophage plaque fecundity

5. Recovery of E. coli O157 strains after exposure to acidification at pH 2

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