SERS-based cascade amplification bioassay protocol of miRNA-21 by using sandwich structure with biotin–streptavidin system

Author:

Liang Zhaoheng12345,Zhou Jun12345ORCID,Petti Lucia6789ORCID,Shao Liyang1011125,Jiang Tao12345ORCID,Qing Yanping1314345,Xie Shusen151617185,Wu Guanyi12345,Mormile Pasquale6789

Affiliation:

1. Institute of Photonics

2. Faculty of Science

3. Ningbo University

4. Ningbo 315211

5. China

6. Institute of Applied Sciences & Intelligent Systems-ISASI

7. CNR

8. 80078 Pozzuoli

9. Italy

10. Department of Electrical and Electronic Engineering

11. Southern University of Science and Technology

12. Shenzhen 518055

13. Affiliated Hospital

14. School of Medicine

15. Key Laboratory of Optoelectronic Science & Technology for Medicine of Ministry of Education

16. Provincial key Laboratory for Photonics Technology

17. Fujian Normal University

18. Fuzhou 350007

Abstract

An ultrasensitive SERS-based cascade amplification bioassay is proposed that couples SERS technology with cascade amplification of the B–S system.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Zhejiang Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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