Three-dimensional imaging on a chip using optofluidics light-sheet fluorescence microscopy-Reference-Cited by-同舟云学术

Three-dimensional imaging on a chip using optofluidics light-sheet fluorescence microscopy

Published:2021 Issue:15 Volume:21 Page:2945-2954
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Vargas-Ordaz Erick J.¹²³⁴⁵,Gorelick Sergey⁶²⁷⁴⁸^ORCID,York Harrison M.⁸²⁷⁴⁹,Liu Bonan¹⁰¹¹²⁴,Halls Michelle L.¹⁰¹¹²⁴,Arumugam Senthil⁸²⁷⁴⁹,Neild Adrian¹²³⁴^ORCID,de Marco Alex⁶²⁷⁴⁸^ORCID,Cadarso Victor J.¹²³⁴⁵

Affiliation:

1. Department of Mechanical and Aerospace Engineering

2. Monash University

3. Clayton

4. Australia

5. Centre to Impact Antimicrobial Resistance – Sustainable Solutions

6. Department of Biochemistry and Molecular Biology

7. 3800 Clayton

8. ARC Centre of Excellence in Advanced Molecular Imaging

9. European Molecular Biology Laboratory (EMBL) Australia

10. Drug Discovery Biology Theme

11. Monash Institute of Pharmaceutical Sciences

Abstract

A novel optofluidic system allows fast 3D imaging of live suspended cells through a sub-μm thick light-sheet formed in PDMS. We demonstrate imaging rates of 120 ms per cell at sub-μm resolution that allow extraction of complex cellular phenotypes.

Funder

Cass Foundation

Monash University

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D1LC00098E

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