Quantifying neutrophil extracellular trap release in a combined infection–inflammation NET-array device

Author:

Datla Udaya Sree123ORCID,Vundurthy Bhaskar4ORCID,Hook Jessica S.5,Menon Nidhi1,Razmi Bagtash Hossein23,Shihabeddin Tarik2,Schmidtke David W.23,Moreland Jessica G.56,Radic Marko Z.7ORCID,Jones Caroline N.23ORCID

Affiliation:

1. Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

2. Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA

3. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA

4. Robotics Institute, Carnegie Mellon University, Pittsburgh, PA, USA

5. Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX, USA

6. Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA

7. Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, USA

Abstract

We present a neutrophil extracellular trap (NET)-array device, engineered to quantify the spatiotemporal dynamics of NET release with single-cell resolution in varied microenvironments simulating infection and inflammation.

Funder

National Institute of General Medical Sciences

Virginia Polytechnic Institute and State University

Publisher

Royal Society of Chemistry (RSC)

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