Interfacing droplet microfluidics with antibody barcodes for multiplexed single-cell protein secretion profiling-Reference-Cited by-同舟云学术

Interfacing droplet microfluidics with antibody barcodes for multiplexed single-cell protein secretion profiling

Published:2021 Issue:24 Volume:21 Page:4823-4830
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Khajvand Tahereh¹^ORCID,Huang Peifeng¹,Li Linmei²,Zhang Mingxia¹,Zhu Fengjiao²,Xu Xing¹,Huang Mengjiao¹,Yang Chaoyong¹³^ORCID,Lu Yao²,Zhu Zhi¹^ORCID

Affiliation:

1. The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

2. Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China

3. Institute of Molecular Medicine, Department of Obstetrics and Gynecology, Renji Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China

Abstract

Multiplexed single-cell protein secretion profiling has been realized on an integrated microfluidic platform. The secreted proteins from each individual cell are captured by specific pre-patterned antibody sites and detected by off-chip ELISA.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Youth Innovation Promotion Association of the Chinese Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D1LC00567G

Reference32 articles.

1. Secretome Analysis of Multiple Pancreatic Cancer Cell Lines Reveals Perturbations of Key Functional Networks