Highly parallel, wash-free, and ultrasensitive centrifugal droplet digital protein detection in sub-microliter blood-Reference-Cited by-同舟云学术

Highly parallel, wash-free, and ultrasensitive centrifugal droplet digital protein detection in sub-microliter blood

Published:2023 Issue:12 Volume:23 Page:2758-2765
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Tang Zhengmin¹²^ORCID,Lv Feifei¹,Reynolds David Eun³,Zhang Shunji⁴,Zheng Shufa¹,Ko Jina³,Chen Yu¹,Wang Yongcheng¹²⁴^ORCID

Affiliation:

1. Department of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

2. Liangzhu Laboratory, Zhejiang University, Hangzhou, China

3. Department of Bioengineering, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, USA

4. College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China

Abstract

A centrifugal droplet digital protein detection technology that combines miniaturized centrifugal droplet generation devices with digital immuno-PCR assays, achieving a femtomolar limit of detection of target proteins in sub-microliters of plasma.

Funder

China Postdoctoral Science Foundation

National Natural Science Foundation of China

Natural Science Foundation of Zhejiang Province

Zhejiang University

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2023/LC/D3LC00205E

Reference44 articles.

1. Protein Detection by Counting Molecules

2. Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations

3. Single Molecule Protein Detection with Attomolar Sensitivity Using Droplet Digital Enzyme-Linked Immunosorbent Assay

4. Ultrasensitive digital quantification of cytokines and bacteria predicts septic shock outcomes

5. Digital Quantification of Proteins and mRNA in Single Mammalian Cells

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