Raman hyperspectral imaging with multivariate analysis for investigating enzyme immobilization-Reference-Cited by-同舟云学术

Raman hyperspectral imaging with multivariate analysis for investigating enzyme immobilization

Published:2020 Issue:23 Volume:145 Page:7571-7581
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Smith Joseph P.¹²³⁴⁵^ORCID,Liu Melinda¹²³⁴⁵^ORCID,Lauro Mackenzie L.¹²³⁴⁵^ORCID,Balasubramanian Madhumitha⁶²³⁴⁵,Forstater Jacob H.⁷²³⁴⁵^ORCID,Grosser Shane T.⁷²³⁴⁵^ORCID,Dance Zachary E. X.¹²³⁴⁵^ORCID,Rhodes Timothy A.⁶²³⁴⁵,Bu Xiaodong¹²³⁴⁵^ORCID,Booksh Karl S.⁸⁹¹⁰¹¹^ORCID

Affiliation:

1. Analytical Research & Development

2. MRL

3. Merck & Co.

4. Inc.

5. Rahway

6. Pharmaceutical Sciences

7. Small Molecule Process Research & Development

8. Department of Chemistry & Biochemistry

9. University of Delaware

10. Newark

11. USA

Abstract

Development of an analytical methodology to study enzyme immobilization for use in directed enzyme evolution and biocatalysis is presented. Raman hyperspectral imaging with PCA successfully resolved enzyme, resin and immobilization-relevant species.

Funder

Merck Sharp and Dohme

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/D0AN01244K

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