Substrate channel evolution of an esterase for the synthesis of cilastatin-Reference-Cited by-同舟云学术

Substrate channel evolution of an esterase for the synthesis of cilastatin

Published:2015 Issue:5 Volume:5 Page:2622-2629
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Luan Zheng-Jiao¹²³⁴,Li Fu-Long¹²³⁴,Dou Shuai¹²³⁴,Chen Qi¹²³⁴,Kong Xu-Dong¹²³⁴,Zhou Jiahai⁵⁶⁷⁸⁴,Yu Hui-Lei¹²³⁴,Xu Jian-He¹²³⁴

Affiliation:

1. State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Centre for Biomanufacturing

2. East China University of Science and Technology

3. Shanghai 200237

4. China

5. State Key Laboratory of Bio-organic and Natural Products Chemistry

6. Shanghai Institute of Organic Chemistry

7. Chinese Academy of Sciences

8. Shanghai 200032

Abstract

Error-prone PCR and site-directed mutagenesis around substrate channel were employed for improving an esterase (RhEst1) activity towards Cilastatin building block. RhEst1_{A147I/V148F/G254A} showed 20 times higher activity than the native enzyme in whole cell biotransformation.

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2015/CY/C5CY00085H

Reference69 articles.

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