Influence of viral transport media and freeze–thaw cycling on the sensitivity of qRT-PCR detection of SARS-CoV-2 nucleic acids-Reference-Cited by-同舟云学术

Influence of viral transport media and freeze–thaw cycling on the sensitivity of qRT-PCR detection of SARS-CoV-2 nucleic acids

Published:2021 Issue:37 Volume:13 Page:15659-15667
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Holohan Cian¹,Hanrahan Sophia¹,Feely Nathan¹,Li Peng²^ORCID,O'Connell John¹,Moss Catherine¹,Carr Michael³⁴,Tagit Oya²,Lee Gil U.¹^ORCID

Affiliation:

1. School of Chemistry and Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Republic of Ireland

2. Magnostics Ltd, Monkstown, Co. Dublin, Republic of Ireland

3. National Virus Reference Laboratory, University College Dublin, Belfield, Dublin, Republic of Ireland

4. International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

Abstract

Synthetic linear SARS-CoV-2 RNA fragments (ssRNA) and a double-stranded DNA plasmid harbouring the SARS-CoV-2 nucleocapsid (N) gene are evaluated in four different VTM for up to 10 freeze–thaw cycles by qRT-PCR following a magnetic bead extraction.

Funder

European Commission

Science Foundation Ireland

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2021/NR/D1NR03933D

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