A colorimetric method for cysteine determination based on the peroxidase-like activity of ficin
Author:
Affiliation:
1. The Key Laboratory on Luminescent and Real-Time Analytical Chemistry
2. Ministry of Education
3. College of Chemistry and Chemical Engineering
4. Southwest University
5. Chongqing 400715
Abstract
H2O2 could oxidize TMB to blue-colored oxTMB with catalysis by ficin. l-Cys competed with TMB for H2O2 leading to fading of the chromogenic reaction.
Funder
National Natural Science Foundation of China
Publisher
Royal Society of Chemistry (RSC)
Subject
General Engineering,General Chemical Engineering,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2018/AY/C8AY00707A
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